The mycobactericidal effect of ATP is reversed by wortmannin and oATP. MDMs, cultured for 5 days, were infected overnight with BCG (MOI of 5:1) and pulsed with ATP (3 mM) for 30 minutes, with or without pre-treatment with wortmannin (100 nM/1 hour) or oATP (0.3 mM/2 hours). The kinetics of BCG viability was determined by 3H-uridine incorporation, monitored at various times post treatment. The figure illustrates the pattern of results obtained from three separate experiments performed in triplicate. The symbols indicate mean values, and vertical bars indicate the standard error. The significance of the various treatment relative to the untreated control are illustrated by: ** = P < 0.005 * = P < 0.05. The results from one 3H-uridine uptake assay are tabulated as mean counts per minute (CPM) data +/- SEM at each time point examined.
Biswas et al. BMC Immunology 2008 9:35 doi:10.1186/1471-2172-9-35