Research article

Galpha_s-coupled receptor signaling actively down-regulates α₄β₁-integrin affinity: A possible mechanism for cell de-adhesion

Alexandre Chigaev^1,2 , Anna Waller² , Or Amit² and Larry A Sklar^1,2

¹Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA

²Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA

author email corresponding author email

BMC Immunology 2008, 9:26doi:10.1186/1471-2172-9-26

Published:	5 June 2008

Abstract

Background

Activation of integrins in response to inside-out signaling serves as a basis for leukocyte arrest on endothelium, and migration of immune cells. Integrin-dependent adhesion is controlled by the conformational state of the molecule (i.e. change in the affinity for the ligand and molecular unbending (extension)), which is regulated by seven-transmembrane Guanine nucleotide binding Protein-Coupled Receptors (GPCRs). α₄β₁-integrin (CD49d/CD29, Very Late Antigen-4, VLA-4) is expressed on leukocytes, hematopoietic stem cells, hematopoietic cancer cells, and others. Affinity and extension of VLA-4 are both rapidly up-regulated by inside-out signaling through several Gα_i-coupled GPCRs. The goal of the current report was to study the effect of Gα_s-coupled GPCRs upon integrin activation.

Results

Using real-time fluorescent ligand binding to assess affinity and a FRET based assay to probe α₄β₁-integrin unbending, we show that two Gα_s-coupled GPCRs (H2-histamine receptor and β2-adrenergic receptor) as well as several cAMP agonists can rapidly down modulate the affinity of VLA-4 activated through two Gα_i-coupled receptors (CXCR4 and FPR) in U937 cells and primary human peripheral blood monocytes. This down-modulation can be blocked by receptor-specific antagonists. The Gα_s-induced responses were not associated with changes in the expression level of the Gα_i-coupled receptors. In contrast, the molecular unbending of VLA-4 was not significantly affected by Gα_s-coupled GPCR signaling. In a VLA-4/VCAM-1-specific myeloid cell adhesion system, inhibition of the VLA-4 affinity change by Gα_s-coupled GPCR had a statistically significant effect upon cell aggregation.

Conclusion

We conclude that Gα_s-coupled GPCRs can rapidly down modulate the affinity state of VLA-4 binding pocket through a cAMP dependent pathway. This plays an essential role in the regulation of cell adhesion. We discuss several possible implications of this described phenomenon.