Table 1 |
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|
Histological evaluation of Mycobacteria-enhanced colitis in IL-10-/- mice |
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|
Treatment/Group |
Number of Mice |
Colitis Disease Score (0 – 12) |
|
|
||
|
Live M. avium paratuberculosis |
10 |
6.12 ± 2.01* |
|
Pasteurized M. avium paratuberculosis |
10 |
4.89 ± 1.41* |
|
Control (vehicle only) |
10 |
1.45 ± 0.79 |
|
Untreated wild-type B6 |
5 |
0 |
|
|
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|
The severity of colitis was monitored by evaluating the histopathological changes in colons of IL-10-/- mice on B6 background that received 200 μl of cream milk or 104 CFU of live or heat-killed M. avium paratuberculosis in cream milk. Following the sacrifice of mice 14 weeks later, colons were fixed, sectioned at 6 μm and stained with hematoxylin and eosin. The sections were microscopically examined and scored for the severity of colitis. The data presented are the mean ± SEM colitis score. Asterisks indicate statistically significant differences, i.e., p < 0.01 between experimental and the control group. |
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Singh et al. BMC Immunology 2008 9:25 doi:10.1186/1471-2172-9-25 |
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