Table 2

Fluorescent antibody panel for the 2002 Haughton Mars Immune Assessment

FITC

PE

ECD

PE/Cy5

APC

CELL POPULATIONS

CD14 (RMO52)

CD45 (J.33)

WBC differential: granulocytes/lymphocytes/monocytes

CD3 (UCHT1)

CD16 (3G8)

CD19 (J4.119)

CD45 (J.33)

Lymphocyte subsets: T cells, B cells and NK cells

CD4 (13B8.2)

CD8 (B9.11)

CD3 (UCHT1)

T cell subsets: CD4+ vs. CD8+

CD45RA (ALB11)

CD45RO (UCHL1)

CD4 (13B8.2)

CD3 (UCHT1)

Naïve/memory CD4+ T cell subsets

CD45RA (ALB11)

CD45RO (UCHL1)

CD8 (B9.11)

CD3 (UCHT1)

Naïve/memory CD8+ T cell subsets

CD8 (B9.11)

CD28 (CD28.2)

CD244 (C1.7)

CD3 (UCHT1)

Cytotoxic effector/suppressor CD8+ T cell subsets

HLA-DR (Immu-357)

CD69 (TP1.55.3)

CD8 (B9.11)

CD4 (13B8.2)

CD3 (UCHT1)

Activation status of T cell subsets (CD4/CD8+)

IFNg* (5344.111)

IL-2* (25723.11)

CD8 (B9.11)

CD3 (UCHT1)

Intracellular detection of cytokines in T cell subsets

All antibodies purchased from either the Beckman-Coulter Corporation except for (*), purchased from Dickinson. The specific antibody clones are indicated in parentheses.

Crucian et al. BMC Immunology 2007 8:7   doi:10.1186/1471-2172-8-7