Figure 9.

Urinary cytokine levels (pg/ml) in response to weekly instillations of BCG, TNF-α, and LPS into the bladder of female mice. Cytokine measurements were performed using fifty microliters of each sample and done in duplicate on the Luminex 100 (Bio-Rad Laboratories, Hercules, CA) using a Bio-Plex Mouse Cytokine 18-Plex (catalog #171-F11181, BioRad). The concentrations of analytes in these assays were quantitated using a standard curve. A regression analysis was performed to derive an equation that was then used to predict the concentration of the unknown samples. Time zero indicates basal levels of cytokines before instillation of any agent. A break at day 1 was added to each graph to contrast the altered cytokine levels when compared to basal levels.