Table 1 |
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|
Comparison of gene expression changes as determined by Affymetrix GeneChip analysis and real time RTPCR |
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|
Gene Symbol |
Log2 Fold Change: Affymetrix |
Log2 Fold Change: Real Time RTPCR |
||
|
|
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|
3M-852A |
3M-011 |
3M-852A |
3M-011 |
|
|
|
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|
IFNA1 |
7.2 |
10.2 |
8.9 |
8.0 |
|
IFNA10 |
5.4 |
6.7 |
7.5 |
7.0 |
|
IFNA13 |
5.5 |
6.6 |
NA |
NA |
|
IFNA14 |
6.7 |
7.9 |
10.2 |
8.2 |
|
IFNA16 |
6.0 |
5.0 |
NA |
NA |
|
IFNA17 |
5.1 |
4.3 |
NA |
NA |
|
IFNA2 |
6.8 |
6.0 |
11.0 |
9.3 |
|
IFNA21 |
7.7 |
6.7 |
9.2 |
8.0 |
|
IFNA4 |
5.9 |
5.1 |
NA |
NA |
|
IFNA5 |
6.3 |
5.8 |
9.8 |
8.6 |
|
IFNA6 |
2.6 |
1.3 |
5.0 |
3.7 |
|
IFNA7 |
5.9 |
4.9 |
NA |
NA |
|
IFNA8 |
2.8 |
2.7 |
8.0 |
7.2 |
|
IFNB1 |
8.0 |
6.2 |
10.4 |
8.6 |
|
Bcl-2 |
1.4 |
1.2 |
3.1 |
2.7 |
|
BCL2L1 |
4.2 |
3.8 |
5.1 |
5.0 |
|
CCL3 |
2.6 |
1.2 |
3.2 |
0.9 |
|
CD80 |
2.5 |
1.8 |
5.6 |
4.9 |
|
CXCL10 |
5.2 |
5.7 |
7.5 |
8.3 |
|
GIP2 |
2.8 |
3.2 |
2.0 |
4.0 |
|
MX1 |
1.4 |
1.6 |
2.3 |
2.4 |
|
MYC |
4.6 |
2.2 |
7.1 |
5.9 |
|
MYD88 |
1.3 |
1.6 |
2.1 |
2.3 |
|
PTGS2 |
3.5 |
1.2 |
5.4 |
3.1 |
|
STAT1 |
1.0 |
1.2 |
1.3 |
1.1 |
|
TNF |
2.4 |
1.4 |
3.2 |
2.2 |
|
|
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|
1Taqman primers and probes for IFNA1 in the real time RTPCR detect both FINA1 and IFNA13. Fold change in expression were calculated with respect to un-stimulated pDC. For the Affymetrix data, values are averages of data sets for four donors for 3M-852A and two donors for 3M-011. For the real time RTPCR data, values are averages of two data sets from two donors for each compound. NA designates not analyzed. |
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|
Birmachu et al. BMC Immunology 2007 8:26 doi:10.1186/1471-2172-8-26 |
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