Figure 9.

Protein regulatory network indicates importance of ISGylation pathway in TLR7-mediated activation of pDC. Protein-interaction network was generated for expression changes in pDC stimulated with 3M-852A and 3M-011 4 hr post-stimulation. The network was generated from a group of genes with the GO ontology classification of ubiquitin cycle using the shortest path algorithm. The network summarizes interactions between the ubiquitin and the ISG15 pathways for protein modification and the genes that are altered in expression upon stimulation of pDC with TLR7 agonists. Remaining network details are as described in Figure 6.