CD4+ CD8- 25+ thymocytes from day 2 thymus are not functional Treg cells. CD4+CD8-CD25+ thymocytes from day 2 newborn thymus or mature Treg cells from adult mice were purified and activated alone or mixed at 1:2 ratio with fixed number of CD4+CD25- responder T cells. Proliferation was determined after 3 days of culture using 3H-thymidine incorporation. Error bars indicate SD of triplicate samples.
Jiang et al. BMC Immunology 2006 7:6 doi:10.1186/1471-2172-7-6