Figure 5.

Antigen-specific stimulation of T cells results in cytokine expression. Intracellular staining with IFNγ FITC/CD69 PE/CD8 PerCPCy5.5/CD3 APC by Ficoll or CPT-processed PBMC from one representative HIV⁺ donor. The numbers displayed on the dot-plots represent the percentage of CD3⁺ and CD8⁺ lymphocytes that are expressing IFNγ and CD69 in response to antigen-specific stimulation or unstimulated control.