p50-/- mice trap IgM-IC less efficiently on FDCs. Localization of IgM-IC in B6 or p50-/- mice was determined by IH 16 h after injection. ER-TR9 identifies marginal zone macrophages (MZM) and FDC-M2 identifies FDCs. IgM-IC (which appear blue in this and all subsequent figures and are identified by arrows) are localized to FDCs in spleens from both groups of mice, but FDCs from B6 mice show more intense staining, indicating that more IgM-IC are trapped in these mice. All IH stains were performed on serial sections and were developed side-by-side for identical periods of time.
Ferguson and Corley BMC Immunology 2005 6:8 doi:10.1186/1471-2172-6-8