Table 6

Inter-experimental reproducibility of the quantification of a range of TGF-β1 and β2-MG RNA standards A set of TGF-β1 and β2-MG RNA standards ranging from 103 to 1010 copies was amplified by RT-PCR. Coefficients of inter-experimental variation were determined from eight different experiments and calculated for threshold cycles.

Absolute copy number

Coefficients of inter-experimental variations (%)


TGF-β1

β2-MG


1.00E+03

1.04

1.20

1.00E+04

1.07

1.19

1.00E+05

0.94

1.13

1.00E+06

0.90

1.08

1.00E+07

0.79

0.95

1.00E+08

0.71

0.91

1.00E+09

0.54

0.93

1.00E+10

0.39

1.18

Average

0.80

1.07


Boeuf et al. BMC Immunology 2005 6:5   doi:10.1186/1471-2172-6-5

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