Table 5 |
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|
Expression stability of endogenous standard genes under non-normalised conditions After 3 hours of stimulation with LPS, PBMC from healthy donors were split in 2 identical aliquots and total cellular RNA was extracted at 2 days interval. The same volume of RNA was reverse transcribed and β2-MG, UBC and YWHAZ transcripts were amplified by real time PCR using CyProQuant-PCR technique. Results show the mean values obtained for the 2 extractions in arbitrary units. |
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|
Stimulation conditions |
Molecules detected (arbitrary units) |
|
|
|
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|
T0 |
LPS 3 h |
|
|
|
||
|
β2-MG |
1.24 107 |
1.48 107 |
|
(1.00 107 – 1.49 107) |
(1.17 107 – 1.79 107) |
|
|
UBC |
2.04 105 |
2.39 105 |
|
(0.62 105 – 3.46 105) |
(1.51 105 – 3.28 105) |
|
|
YWHAZ |
1.89 105 |
2.80 105 |
|
(0.78 105 – 3.01 105) |
(2.25 105 – 3.34 105) |
|
|
|
||
|
Boeuf et al. BMC Immunology 2005 6:5 doi:10.1186/1471-2172-6-5 |
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