Table 4

RNA and DNA standard-based quantification of TNF-a, IL-1β and IFN-γ transcripts Fifty million copies of TNF-α, IL-1β and IFN-γ standard RNA were amplified in parallel of a range of RNA standard (CyProQuant-PCR) and DNA standard. Copy numbers were directly deduced from the cycle at threshold. *For TNF-α, the experiment was repeated at two days interval.

Target gene

Actual copy number

Calculated copy number


CyProQuant-PCR

DNA standard approach


TNF-α*

5 107

5.2 107

7.7 105

5 107

5.0 107

3.6 105

IL-1β

5 107

4.3 107

6.7 105

IFN-γ

5 107

5.5 107

7.8 105


Boeuf et al. BMC Immunology 2005 6:5   doi:10.1186/1471-2172-6-5

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