Table 2

Comparison between CyProQuant-PCR and DNA standard-based approaches Efficiencies (E) are deducted from the slopes (S) of the standard curves based on E = 100*(10-1/S - 1). The deducted RT efficiency from the CyProQuant-PCR is calculated by dividing the RT-PCR efficiency from the RNA range (ERT-PCR) by the PCR efficiency from the cDNA range (EPCR).

Target gene

CyProQuant-PCR

DNA standard-based PCR


RNA range

cDNA range

Deducted RT efficiency

DNA range



Slope

ERT-PCR

Slope

EPCR

Slope

EPCR


TNF-α

-3.610

89

-3.556

91

98

-3.503

93

IL-1β

-3.479

94

-3.197

105

89

-3.067

112

IFN-γ

-3.843

82

-3.116

109

75

-2.944

119


Boeuf et al. BMC Immunology 2005 6:5   doi:10.1186/1471-2172-6-5

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