Methodology article
Standardization of cytokine flow cytometry assays
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* Corresponding author: Holden T Maecker holden_maecker@bd.com
1 BD Biosciences, San Jose, USA
2 Université de Montreal and CANVAC, the Canadian Network for Vaccines and Immunotherapeutics, Montreal, Canada
3 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA
4 Chelsea and Westminster Hospital and IAVI, London, UK
5 Uganda Virus Research Institute and IAVI, Entebbe, Uganda
6 Kenya AIDS Vaccine Initiative (KAVI), University of Nairobi, Kenya
7 Centre Hospitalier Universitaire Vaudois and EUROVAC, Lausanne, Switzerland
8 University of Washington and HVTN, Fred Hutchinson Cancer Research Center, Seattle, USA
9 Duke University Medical Center and HVTN, Durham, USA
10 Vaccine Research Center, National Institutes of Health, Bethesda, USA
11 University of California, San Francisco, USA
12 Merck and Co., West Point, USA
13 University of Pennsylvania, Philadelphia, USA
14 Sanofi Pasteur, Lyon, France
15 Massachusetts General Hospital, Boston, USA
16 National Institute for Communicable Diseases, Johannesburg, South Africa
17 Henry Jackson Foundation, Rockville, USA
BMC Immunology 2005, 6:13 doi:10.1186/1471-2172-6-13
Published: 24 June 2005Additional files
Additional File 1:
Protocol for fixed, activated whole blood (Experiment 1)
Format: PDF Size: 234KB Download file
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Additional File 2:
Protocol for shipped whole blood (Experiment 2)
Format: PDF Size: 268KB Download file
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Additional File 3:
Protocol for cryopreserved PBMC (Experiment 3)
Format: PDF Size: 337KB Download file
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Additional File 4:
Protocol for cryopreserved PBMC with lyophilised antigen and antibody plates (Experiment 4)
Format: PDF Size: 136KB Download file
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