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Characteristics of different parallel plate flow chambers |
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| Classic |
GlycoTech |
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| Single-Pass |
Single-Pass |
Re-circulation |
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| Chamber |
Chamber |
Chamber |
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|
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| Cells Needed For |
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| Shear Curve |
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| 0.062" I.D. |
1 × 107 cells |
2.4 × 106 cells |
N/A |
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| (5,000 ul) |
(1,175 ul) |
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| 0.02" I.D. |
9.9 × 106 cells |
2 × 106 cells |
N/A |
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| (4,950 ul) |
(1,000 ul) |
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| Leucocytes Per |
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| Constant Shear |
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| Experiment* |
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| 0.062" I.D. |
1.2 × 107 cells |
2.2 × 106 cells |
4.1 × 106 cells |
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| (6,000 ul) |
(1,075 ul) |
(2,070 ul) |
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| 0.02" I.D. |
9.9 × 106 cells |
1.8 × 106 cells |
1.7 × 106 cells |
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| (4,950 ul) |
(900 ul) |
(850 ul) |
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| Dead Volume |
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| 0.062" I.D. |
1,500 ul |
225 ul |
2,070 ul |
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| 0.04" I.D. |
820 ul |
110 ul |
1,290 ul |
|
| 0.02" I.D. |
450 ul |
50 ul |
850 ul |
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| 0.01" I.D. |
360 ul |
35 ul |
740 ul |
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| Number Of Constant |
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| Shear Experiments |
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| Per Cell |
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| Preparation* |
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| Neutrophil |
2 |
15 |
23 |
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| Eosinophil |
0 |
4 |
5 |
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| Basophil |
0 |
2 |
4 |
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| Reduction In Cell/ |
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| mAb/Reagent Use |
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| 0.062" I.D. |
0% |
82% |
66% |
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| 0.02" I.D. |
0% |
82% |
83% |
|
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* Number of experimental conditions calculated assuming 0.02" I.D. for the improved chamber and 0.062" I.D. for the classic chamber. I.D., internal diameter; N/A, not applicable, see text for details. Approximate cell number per preparation is 3 × 107 neutrophils, 2 × 106 eosinophils, and 6 × 105 basophils per preparation. Dead volume refers to the entire fluid volume external to the flow path. | ||||
Brown and Larson BMC Immunology 2001 2:9 doi:10.1186/1471-2172-2-9 |
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