Figure 2.

Comparison of measurements in the 3 parallel plate flow chamber configurations. (A) Shear curves generated by perfusing neutrophils over activated with LPS (squares) or unactivated (triangles) EC using the classic (closed triangle and square) or single pass (open triangle or square) Glycotech chambers. The experiment was performed as described in the material and methods section. (B) Constant shear experiments comparing the three different chamber configurations as described in material and methods. Neutrophils were perfused over activated endothelium in either the classic (B1), single pass Glycotech (B2), or recirculation chambers (B3). The number of rolling (black bars) and arrested (clear bars) cells were determined each minute. S.D. is indicated by bars. S.D. bars directed up represent S.D. for rolling cells, and S.D. bars directed down represent S.D. for firm cells; n = 3.