Table III

Secreted IFN-γ and TNF-α from T Cell Culture*

Source

Cells

IFN-γ (pg/ml)

TNF-α (pg/ml)


Aged

PBMC

70,800 ± 8,500 (p < 0.001)

35,400 ± 6,200 (p < 0.005)

Control

PBMC

20,300 ± 3,000

16,900 ± 4,100

Aged

CD8+

86,300 ± 15,600 (p < 0.001)

44,200 ± 7,800 (p < 0.005)

Control

CD8+

24,400 ± 6,200

19,400 ± 4,300

HIV+

CD8+

121,000 ± 15,600 (p < 0.001)

57,200 ≅ 11,500(p < 0.001)

Control

CD8+

27,300 ± 4,800

16,400 ± 4,900


PBMC and purified CD8+ T cells were incubated for 66 hrs. as described in Methods, and the media was tested by ELISA. The results are expressed in pg IFN-γ or TNF-α per 105 cells (mean value ± S.D.). The standard deviation is shown and the p valves, determined with Student's t test, compare the IFN-γ or TNF-α secreted by aged or HIV+-derived cells and normal control cells. Eight different aged and HIV+ donors were used, and seven young control donors. In the presence of 5 mM of N-acetylcysteine, the production of both INF-γ and TNF-α was significantly increased in aged, HIV+ and control samples, generally by 1.5 to 2 fold.

Eylar et al. BMC Immunology 2001 2:10   doi:10.1186/1471-2172-2-10

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