Table II

Comparison of cytokine Production in CD8+ CD28 and CD8+ CD28+ T cells

Cells derived form

HIV+

Control

Aged

Control


In TNF-α studies

% CD28- of the

68.3

36.3

64.2

27.1

total CD8+ cells

% CD28-

55.2

26.8

68.0

29.5

producing TNF-α

% Total TNF-α±

79.5

20.5

84.6

15.4

In IFN-γ studies

% CD28- of the total

70.0

30.7

61.3

28.0

CD8+ cells

% CD28- producing

59.8

21.2

62.3

35.1

IFN-γ

% Total IFN-γ ±

86.7

13.3

79.6

20.4


*Analysis of CD8+ T cells from CD28+ and CD28- subsets in terms of cytokine production was performed by flow cytometry as described in Methods. Twenty HIV+ and aged donors and fifteen control donors were used in the IFN-γ studies; twenty-two and nineteen were used respectively in the TNF-α studies. ± The percent total of TNF-α or IFN-γ refers to the percentage of CD28- cells producing cytokine compared to the total produced by the controls and HIV+ (or aged) CD28- T cells. For example, in the TNF-α studies, 8% of the control CD28- cells produce cytokine (27.1 × 29.5), whereas in the aged state, CD28- cells producing TNF-α is 43.7% (64.2 × 68.0). Thus the TNF-α produced by aged CD28- CD84 T cells is 84.6% compared to 15.4% for controls.

Eylar et al. BMC Immunology 2001 2:10   doi:10.1186/1471-2172-2-10

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