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Open Access Research article

Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs

Yoshihito Suda1, Julio Villena27, Yu Takahashi1, Shoichi Hosoya2, Yohsuke Tomosada2, Kohichiro Tsukida2, Tomoyuki Shimazu3, Hisashi Aso4, Masanori Tohno5, Mitsuharu Ishida1, Seiya Makino6, Shuji Ikegami6 and Haruki Kitazawa2*

Author Affiliations

1 Department of Food, Agriculture and Environment, Miyagi University, Sendai, Japan

2 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science Tohoku University, 981-8555 Sendai, Japan

3 Laboratory of Animal Breading and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan

4 Cell Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan

5 National Institute of Livestock and Grassland Science, Nasushiobara, Japan

6 Division of Research and Development, Meiji Co., Ltd, Kanagawa, Japan

7 Laboratory of Immunobiotechnology, CERELA-CONICET, Tucuman, Argentina

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BMC Immunology 2014, 15:24  doi:10.1186/1471-2172-15-24

Published: 19 June 2014

Abstract

Background

Immunoregulatory probiotics (immunobiotics) have been proposed to improve piglets’ immune system to avoid intestinal infections and reduce unproductive inflammation after weaning. Previously, it was demonstrated that Lactobacillus jensenii TL2937 (LjTL2937) attenuated the inflammatory response triggered by activation of Toll-like receptor 4 (TLR-4) in porcine intestinal epithelial (PIE) cells and antigen presenting cells (APCs) from porcine Peyer’s patches (PP).

Objective

In view of the critical importance of PIE-APCs interactions in the regulation of intestinal immune responses, we aimed to examine the effect of LjTL2937 on activation patterns of APCs from swine PPs in co-cultures with PIE cells. In addition, we investigated whether LjTL2937 was able to beneficially modulate intestinal immunity of piglets after weaning to improve immune-health status.

Results

Stimulation of PIE-APCs co-cultures with LjTL2937 increased the expression of MHC-II, CD80/86, IL-10, and Bcl-3 in CD172a+CD11R1- and CD172a+CD11R1high APCs. In addition, the TL2937 strain caused the upregulation of three negative regulators of TLR4 in PIE cells: MKP-1, Bcl-3 and A20. These changes significantly reduced the inflammatory response triggered by TLR4 activation in PIE-APCs co-cultures. The in vivo experiments using castrated male piglets (crossbreeding (LWD) with Landrace (L), Large Yorkshire (W) and Duroc (D))of 3 weeks of age demonstrated that feeding with LjTL2937 significantly reduced blood complement activity and C reactive protein concentrations while no changes were observed in blood leukocytes, ratio of granulocytes to lymphocyte numbers, macrophages’ activity and antibody levels. In addition, treatment with LjTL2937 significantly improved growth performance and productivity, and increased carcass quality.

Conclusions

We demonstrated that the use of immunobiotics strains like LjTL2937, as supplemental additives for piglets feedings, could be used as a strategy to maintain and improve intestinal homeostasis; that is important for the development of the pig and for health and performance throughout the productive life of the animal.

Keywords:
Immunobiotics; Immune performance; Productivity; Piglets; Lactobacillus jensenii TL2937; TLR4; TLRs negative regulators