Open Access Research article

T-cell activation discriminates subclasses of symptomatic primary humoral immunodeficiency diseases in adults

Marie-Quitterie Picat1234, Rodolphe Thiébaut1234*, François Lifermann5, Xavier Delbrel6, Daniel Adoue7, Linda Wittkop1234, Anne-Laure Fauchais8, Patrick Rispal9, Jean-François Moreau101112 and Jean-François Viallard1314*

Author Affiliations

1 INSERM, ISPED, Centre INSERM U897-Epidemiologie-Biostatistique, Bordeaux F-33076, France

2 University of Bordeaux, ISPED, Centre INSERM U897-Epidemiologie-Biostatistique, Bordeaux F-33076, France

3 Department of Medical Information, Bordeaux University Hospital, Bordeaux F-33000, France

4 INRIA SISTM, F-33405 Talence, France

5 Department of Internal Medicine, Dax Hospital, Dax F-40107, France

6 Department of Internal Medicine, Pau Hospital, Pau F-64046, France

7 Department of Internal Medicine, Purpan Hospital, Toulouse F-31059, France

8 Department of Internal Medicine, Dupuytren Hospital, Limoges F-87042, France

9 Department of Internal Medicine, Agen Hospital, Agen F-47923, France

10 University of Bordeaux, Bordeaux F-33076, France

11 CNRS, UMR 5164, Bordeaux F-33076, France

12 Bordeaux University Hospital, Laboratory of Immunology, Bordeaux F-33076, France

13 Department of Internal Medicine and Infectious Diseases, Bordeaux University Hospital, Pessac F-33604, France

14 Hôpital Haut-Lévêque - Service de médecine interne, 5, avenue de Magellan, Pessac 33604, France

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BMC Immunology 2014, 15:13  doi:10.1186/1471-2172-15-13

Published: 12 March 2014

Additional files

Additional file 1: Table S1:

References of the panels of monoclonal antibodies used for identifying cells by flow cytometry using a FC500 flow cytometer from Beckman-Coulter.

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Additional file 2: Figure S1:

Gating strategies for for flow cytometric assessment of activated T-lymphocytes and other major lymphocyte sub-populations in the blood on a CVID patient.

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Additional file 3: Figure S2:

Immunological markers of 12 healthy donors, plotted on the first two principal components.

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Additional file 4: Table S2:

Immunological marker values of normal patients.

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Additional file 5: Figure S3:

Thirty-eight immunological markers involved in PHID on the two first principal components. ALTADIH Cohort, 2007-2010.

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Additional file 6: Figure S4:

Classification of 55/57 CVID patients by hierarchical cluster analysis, according to 23 immunological markers involved in the disease. ALTADIH Cohort, 2007-2010.

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Additional file 7: Table S3:

Sensibility [95% confidence interval] and specificity [95% confidence interval] of the model EUROclass to determine CVID complications with a probability ≥ 50% or < 50%.

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Additional file 8: Table S4:

Sensibility [95% confidence interval] and specificity [95% confidence interval] of the model EUROclass and CD8+HLA-DR+ to determine CVID complications with a probability ≥ 50% or < 50%.

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