Table 1

Summary of the percentage of cells expressing selected cytokines as determined by flow cytometry. Response (fold increase) of CD4+ and CD8+ lymphocytes from rBCG-immunized mice (rBCG018, rBCG032) was compared to those of BCG-immunized mice (BCG).

CD4+/IL-2

Ag85B (P1)

Ag85B (P2)

Ag85B (P3)

Mtb8.4

ESAT-6

CFP10

MTP40 (P1)

MTP40 (P2)


BCG

rBCG018

rBCG032

6.7

11.5

12.5

7.4

20.6*

16.5*

4.8

11.4*

6.5

3.8

12.5**

8.6*

6.1

3.6

5.6

3.1

2.4

8.4*

4.6

3.3

6.4

6.3

4.5

5.3


CD4+/IL-4


BCG

rBCG018

rBCG032

8.5

10.1

10.4

9.3

7.7

15.8

8.5

16.9

18.4*

9.4

8.4

13.8

3.5

1.1

3.8

5.4

3.5

5.7

8.4

7.8

8.6

5.9

5.7

8.4


CD4+/IFN-γ


BCG

rBCG018

rBCG032

4.6

17.4**

18.4**

8.0

21.5*

22.5*

6.4

16.6*

8.4

3.7

8.9*

7.5*

5.4

5.5

7.4

5.7

2.5

5.7

5.6

2.7

9.0

3.8

2.7

9.7*


CD8+/IL-2


BCG

rBCG018

rBCG032

12.3

21.5

25.0*

9.4

34.3**

30.5**

2.1

36.3**

30.4**

2.7

10.4**

9.8**

5.3

5.2

19.4**

3.6

6.4

18.3**

9.4

8.7

19.3*

4.7

5.5

7.4


CD8+/IL-4


BCG

rBCG018

rBCG032

5.5

16.5**

20.8**

3.7

15.5**

12.5**

7.8

16.5*

20.5*

5.7

8.4

13.8*

4.8

7.5

13.8*

4.6

8.6

11.4*

6.4

8.0

11.5

6.0

7.3

7.4


CD8+/IFN-γ


BCG

rBCG018

rBCG032

5.5

20.5**

25.4**

8.7

20.5*

24.5*

6.5

19.5**

21.5**

3.8

10.5*

9.7*

9.7

13.2

15.4

4.7

10.4*

11.3*

8.4

9.9

14.3

5.9

7.7

9.5


** ≥ 3 fold increase (high response) * ≥ 2 fold increase (moderate response) ≤ 2 fold increase (weak to no response)

Mohamud et al. BMC Immunology 2013 14(Suppl 1):S5   doi:10.1186/1471-2172-14-S1-S5

Open Data