Table 1

Immunogenicity AFCo1 obtained by CFS and administered by the i.n route: Anti-PLn IgA, IgG and IgG isotypes measured by ELISA

Groups

IgG(U/mL) sera

IgA(AU/mL) saliva

IgG1(OD) sera

IgG2a(OD) sera

IFNγ(pg/mL)


AFCo1

4415.74*

2147.03*

1.378*

1.62*

2787.04*

Placebo

106.05

101.48

0.102

0.120

162.381

PHA

-

-

-

-

3065.34*


Groups of female BALB/c mice (n = 5) were immunized with AFCo1 by the i.n route (3 doses, 12.5µL in each nostril) with a 7 day interval. Anti-PLn IgA in saliva or serum and anti-PLn IgG and IgG subclass induction by AFCo1 and IFN-γ production by spleen cells from immunized mice. Phosphate Buffer was used for the Placebo group. IFN-γ concentration was evaluated in splenocyte supernatants, 7 days after the last dose and stimulated in vitro with PLn or phytohaemagglutinin (PHA). Asterisks denote significant differences between groups * p<0.05.

Zayas et al. BMC Immunology 2013 14(Suppl 1):S4   doi:10.1186/1471-2172-14-S1-S4

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