This article is part of the supplement: Proceedings of Delivery Systems and Current Strategies to Drug Design

Open Access Proceedings

Passive administration of purified secretory IgA from human colostrum induces protection against Mycobacterium tuberculosis in a murine model of progressive pulmonary infection

Nadine Alvarez1, Oscar Otero1, Frank Camacho1, Reinier Borrero1, Yanely Tirado1, Alina Puig1, Alicia Aguilar1, Cesar Rivas2, Axel Cervantes2, Gustavo Falero-Díaz1, Armando Cádiz3, María E Sarmiento1, Mohd Nor Norazmi45, Rogelio Hernández-Pando2 and Armando Acosta1*

Author affiliations

1 Department of Molecular Biology. Finlay Institute. Center of Research – Producction of Vaccines. Ave. 27 No. 19805, La Lisa. Ciudad de la Habana, Cuba. AP. 16017, CP 11600

2 Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition “Salvador Zubiràn”, D.F. Mexico. CP 14 000

3 Enterprise of Production of Serum and Hemoderivates “Adalberto Pesant González”. Ave 51 No.33 235 km 19 medio ½. Arroyo Arenas, La Lisa. Ciudad de la Habana, Cuba. CP 13400

4 School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Malaysia

5 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kubang Kerian, Malaysia

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Citation and License

BMC Immunology 2013, 14(Suppl 1):S3  doi:10.1186/1471-2172-14-S1-S3

Published: 25 February 2013

Abstract

Background

Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as colostrum, breast milk, tears and saliva. The high concentration of human secretory IgA (hsIgA) in human colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections.

Materials and methods

Human secretory IgA was purified from colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated.

Results

The passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury.

Conclusions

HsIgA purified from colostrum protects against M. tuberculosis infection in an experimental mouse model.