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This article is part of the supplement: Proceedings of Delivery Systems and Current Strategies to Drug Design

Open Access Proceedings

Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac2SGL complexes from Mycobacterium tuberculosis-infected cells

Frank Camacho1, Jim Huggett2, Louise Kim2, Juan F Infante1, Marco Lepore3, Viviana Perez1, María E Sarmiento1, Graham Rook2 and Armando Acosta1*

Author affiliations

1 Finlay Institute, Havana, Cuba

2 UCL, London, UK

3 Experimental Immunology, University Hospital, Basel, Switzerland

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Citation and License

BMC Immunology 2013, 14(Suppl 1):S2  doi:10.1186/1471-2172-14-S1-S2

Published: 25 February 2013

Abstract

The development of molecules specific for M. tuberculosis-infected cells has important implications, as these tools may facilitate understanding of the mechanisms regulating host pathogen interactions in vivo. In addition, development of new tools capable to targeting M. tuberculosis-infected cells may have potential applications to diagnosis, treatment, and prevention of tuberculosis (TB). Due to the lack of CD1b polymorphism, M. tuberculosis lipid-CD1b complexes could be considered as universal tuberculosis infection markers. The aim of the present study was to display on the PIII surface protein of m13 phage, a human αβ single-chain T-cell receptor molecule specific for CD1b:2-stearoyl-3-hydroxyphthioceranoyl-2´-sulfate-α-α´-D-trehalose (Ac2SGL) which is a complex presented by human cells infected with M. tuberculosis. The results showed the pIII fusion particle was successfully displayed on the phage surface. The study of the recognition of the recombinant phage in ELISA and immunohistochemistry showed the recognition of CD1b:Ac2SGL complexes and cells in human lung tissue from a tuberculosis patient respectively, suggesting the specific recognition of the lipid-CD1b complex.