Antimeningococcal protectogenic potential and adjuvant capacity of the new proteoliposomic (NPL) formulation from N. meningitidis serogroup B, without aluminum hydroxide. A: Micrograph obtained by TEM, showing abundant spherical particles of a size ranging between 50 and 70 nm of diameter. B Diagram showing the scheme of immunization designed for the evaluation of the formulations in BALB/c mice. In C, the graph represents the mean and SD of the level of specific antibodies expressed in units of IgG anti OMV in the serum of the immunized mice at 7, 14, 21 and 60 days, after the last dose of immunization, (*) indicate significant differences for p< 0.05. The results of the determination of the bactericidal activity of serum are shown in D, each bar represents the mean of three individual experiments, accomplished for each group of assay, at the 21 days after the last immunization dose and at the 60 days after receiving the antigenic intradermical challenge for DTH assay. NPL: Mice inoculated with the formulation of neo-proteoliposomes, PD-Tp: Mice immunized with the protein complex of the N. meningitidis serogroup B (PD) in buffer (Tris HCL 5mM, 0.1% detergent, pH 7.4), VA-MENGOC-BC®: Mice inoculated with the Cuban antimeningococcal BC vaccine, OMV: Mice inoculated with Outer Membrane Vesicles of the N. meningitidis serogroup B, NC: Control group that received buffer solution. IM: Intramuscular administration route, IN: Intranasal administration route.
Tamargo et al. BMC Immunology 2013 14(Suppl 1):S12 doi:10.1186/1471-2172-14-S1-S12