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This article is part of the supplement: Proceedings of Delivery Systems and Current Strategies to Drug Design

Open Access Proceedings

Evaluation of specific humoral immune response and cross reactivity against Mycobacterium tuberculosis antigens induced in mice immunized with liposomes composed of total lipids extracted from Mycobacterium smegmatis

María de los Angeles García1*, Reinier Borrero1, Reynel Marrón1, María E Lanio2, Lien Canet2, Oscar Otero1, Ramlah Kadir3, Siti Suraiya4, Caridad Zayas1, Yamilé López1, Mohd Nor Norazmi35, Maria E Sarmiento1 and Armando Acosta1

Author affiliations

1 Molecular Biology Department Finlay Institute. Ave. 27 No. 19805, La Lisa. La Havana, Cuba. AP. 16017, CP11600

2 Center for Protein Studies, Faculty of Biology, Havana University, Cuba

3 School of Health Sciences, Universiti Sains Malaysia, Malaysia

4 School of Medical Sciences, Universiti Sains Malaysia, Malaysia

5 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Malaysia

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Citation and License

BMC Immunology 2013, 14(Suppl 1):S11  doi:10.1186/1471-2172-14-S1-S11

Published: 25 February 2013

Abstract

The development of a new tuberculosis (TB) vaccine has become one of the main objectives of the scientific community. Protein antigens have been widely explored as subunit TB vaccines, however lipid antigens could be equally important to be used or included in such a vaccine. The aim of this study was to demonstrate the potential of a liposome formulation composed of an extract of lipids from Mycobacterium smegmatis (Ms) as a TB vaccine candidate. We evaluated the immunogenicity of this formulation as well as the cross reactive response against antigens from Mycobacterium tuberculosis (MTb) in BALB/c mice. We determined the anti-liposome IgG response in sera from TB patients and from healthy subjects who displayed a positive (PPD+) or negative (PPD-) tuberculin skin test. A significant increase in anti-liposome IgG (p<0.05) was detected in animals immunized with Bacille Calmette-Guérin (BCG) compared with all groups, and in the group immunized with liposomes from Ms (LMs) compared to animals immunized with either LMs adjuvanted with aluminium (LMs-A) or the negative control group (phosphate buffered saline, PBS) respectively. With respect to the cross reactive response against a cocktail of cell wall antigens (CWA) from MTb, significantly higher IgG levels were observed in animals immunized with BCG and LMs compared to negative controls and either, aluminium-adjuvanted liposomes (LMs-A) or montanide (LMs-M) (p<0.05). Furthermore, the anti-liposome IgG response was significantly superior in sera from pulmonary TB patients compared to PPD+ and PPD- healthy subjects (p<0.001) suggesting the expression of these antigens in vivo during active MTb infection. The results obtained provide some evidence for the potential use of liposomes containing total lipid extracts of Ms as a TB vaccine candidate.