Characterization of PLc derived from V. cholerae O1. Micrograph in A was obtained by TEM and shows vesicle shape of PLc. B shows the main antigens identified in PLc by Western Blot: Lanes 1 to 3 represent blotted PLc (5 μg) and Lane 4 the biotinylated molecular weight pattern. Each Line was treated with different Mabs. (Lane 1): MAb 2F12F1 (anti MSHA), (Lane 2): MAb 9H12E6 (anti OmpU) and (Lane 3): MAb 2B4G5 (anti O-p-LPS Ogawa). Band centered for LPS is ~30 kDa (Lane 3), OmpU~38 kDa (Lane 2) and MSHA~17 kDa (Lane 1).
Acevedo et al. BMC Immunology 2013 14(Suppl 1):S10 doi:10.1186/1471-2172-14-S1-S10