Figure 5.

In vitro effects of budesonide on the antibacterial host defense of airway epithelium cells. A) MLE-12 cells were pretreated with budesonide, infected with GFP-labeled P. aeruginosa and observed by confocal microscopy (original magnification ×400). B) MLE-12 cells were lysed and plated to confirm the presence of intracellular bacteria. Bacteria were counted using drop plates. C) CRAMP expression in MLE-12 cells was determined by western blot analysis. D) MLE-12 cells were pretreated with CRAMP neutralizing antibody. MLE-12 cells were lysed and plated to confirm the presence of intracellular bacteria. Bacteria were counted using drop plates. E) MLE-12 cells were pretreated with CRAMP neutralizing antibody. GFP-labeled P. aeruginosa were observed by confocal microscopy (original magnification ×400). F) Effects of budesonide on respiratory infection due to P. aeruginosa.

Wang et al. BMC Immunology 2013 14:7   doi:10.1186/1471-2172-14-7
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