Expression of CRAMP in lungs by immunohistochemistry and western blot analysis. A) CRAMP expression and localization in lung tissues was determined by immunostaining (original magnification ×200). B) CRAMP expression in lung tissue was determined by western blot analysis. C) Densitometry analysis of CRAMP in lung tissues. Both immunohistochemistry and western blot analysis showed that expression of CRAMP in OVA/Bud/P.a mice was markedly lower than in OVA/P.a mice. CRAMP expression in PBS control mice was significantly lower than in OVA/No, OVA/P.a and OVA/Bud/P.a mice.
Wang et al. BMC Immunology 2013 14:7 doi:10.1186/1471-2172-14-7