Effects of budesonide on lung infection in mice exposed to P. aeruginosa. A) Animal model. Mice were sensitized by intraperitoneal injection with ovalbumin (OVA) in aluminum hydroxide on days 1 and 8. From days 15 through 22, mice were intranasally challenged with OVA. OVA-challenged mice were treated with inhaled budesonide on days 23 through 30. On day 31, budesonide-treated mice were infected intranasally with 1 x 107 CFU ml-1P. aeruginosa. Mice were killed after 24 h of P. aeruginosa infection. B) Lung samples were stained with hematoxylin and eosin (HE) (original magnification ×200). OVA/Bud mice had less extensive infiltration of inflammatory cells in the airways than OVA/No mice. However, OVA/Bud/P.a mice developed more inflammation and cellular infiltration than OVA/P.a mice.
Wang et al. BMC Immunology 2013 14:7 doi:10.1186/1471-2172-14-7