Morphological characteristics of cultured macrophages derived from spleen (A, D), bone marrow (B, E) and peritoneal cavity (C, F), and their cell size assessment (G). All cells were cultured in complete RPMI1640 on 6-well plates, and after removal of supernatant, cells were then stained with Giemsa-wright dye (A, B, C) and to demonstrate lysosome, anti-LAMP1 (D, E, F) (original magnification x400). Cell size was assessed by flow cytometry analysis (G).
Wang et al. BMC Immunology 2013 14:6 doi:10.1186/1471-2172-14-6