ProTα- or proTα(100–109)-matured DCs secrete pro-inflammatory cytokines. Culture supernatants from iDCs and DCs matured with LPS, TNF-α, proTα or proTα(100–109) for 48 h were assessed for their TNF-α, IL-12 and IL-10 content. iDCs were treated (+) or not (−) with an anti-TLR-4 mAb (a-TLR-4) for 1 h prior to their maturation. Data are given as mean values ± SDs from 3 donors. * p < 0.05.
Ioannou et al. BMC Immunology 2013 14:43 doi:10.1186/1471-2172-14-43