Additional file 2: Figure S2.
Analysis of cell population responsible for the protective effects by targeted vaccine. Animals (5 mice per group) were vaccinated with scFvNLDC-145-HER2 on days -21 and -7. On day 0, mice were inoculated s.c. with D2F2/E2 tumor cells. For in vivo depletion of CD4+, CD8+ T cells or CD19+ B cells, an anti-CD4 (0.5 mg/mouse; Clone GK1.5), anti-CD8 (0.5 mg/mouse; Clone YTS 169.4), anti-CD19 (0.2 mg/mouse; Clone 1D3) or control (0.5 mg/mouse; Clone 2A3) mAb was injected i.p. on days -7, -3 and -1 and repeated weekly later. All mAbs were purchased from BioXcell. Tumor developments were monitored, and animal survival was calculated.
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Cao et al. BMC Immunology 2013 14:39 doi:10.1186/1471-2172-14-39