Additional file 1: Figure S1.

Construction and expression of pcDNA3.1-scFvNLDC-145-EGFP and pcDNA3.1-EGFP a generated pcDNA3.1-scFvNLDC-145-EGFP by replacing the HER2 fragment with EGFP sequence cloned from pEGFP-N1 plasmid. The pcDNA3.1 vector encoding EGFP without DC-targeting scFv fragment as control. b 293T cells grown in 24-well plate were transfected with the two expression vectors using Lipofectamine 2000 (invitrogen). Green fluorescent protein GFP was observed by inverted fluorescence microscope (X51-A21PH, OLYMPUS).

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Cao et al. BMC Immunology 2013 14:39   doi:10.1186/1471-2172-14-39