IL-17R expresses in NSCs. Dissociated single cells were incubated in stem cell culture or differentiation medium in 12-well plates for 2 days and processed for immunofluorescence. (a) Immunocytochemistry images of neural stem cells cultures labeled for IL-17R (green) and counterstained with the nuclei dye DAPI (blue). (b) IL-17R antibody was omitted to serve as control. Magnification ×20 for A, ×20 for B. (c) Plot depicts the percentage of IL-17R positive cells. (d) RT-PCR for IL-17R expression in NSCs. Primary oligodendrocytes (>93% GalC+) of wild type B6 mice served as positive control and of IL-17R knockout mice as negative control. Mean percentages were determined from five independent experiments. WT: wild type.
Li et al. BMC Immunology 2013 14:20 doi:10.1186/1471-2172-14-20