Reaction of anti-B. tropicalis, recombinant allergen IgE antibodies and total IgE to A. lumbricoides extract. Twenty adults' sera with anti-B. tropicalis extract IgE antibodies were pre-incubated with the indicated concentrations of A. lumbricoides crude extract and tested in indirect ELISAs using B. tropicalis crude extract (BtE) or recombinant allergens (rBlo t 5 and rBlo t 21) as antigens or assayed for total IgE levels. The reductions in anti-Bt, anti-rBlo t 5, and anti-rBlo t 21 IgE antibody levels or total IgE levels were calculated as described in the Methods section (A), * p < 0.0231, ** p < 0.0020 and *** p < 0.0008; Wilcoxon signed rank test. (B) Specific sIgE levels in sera non-adsorved and adsorved with Ascaris lumbricoides extract (OD 450 nm), * p < 0.0217 ** p < 0.0012, # p < 0.0030, ## p< 0.0041; Wilcoxon signed rank test.
Carvalho et al. BMC Immunology 2013 14:11 doi:10.1186/1471-2172-14-11