Figure 1.

Expression and purification of recombinant proteins rBlo t 5 and rBlo t 21. The his-tagged proteins rBlo t 5 (A) and rBlo t 21 (B) were induced by IPTG and affinity-purified using the resin Ni-NTA agarose. The samples collected during expression induction and purification were analyzed by 15% SDS-PAGE stained with Coomassie Blue. Arrows indicate purified proteins. (C) Immunoblotting of the rBlo t 5 (A) and rBlo t 21 with negative control sera (Ns) and positive sera – asthmatic patient (Ps). MW, molecular weight.

Carvalho et al. BMC Immunology 2013 14:11   doi:10.1186/1471-2172-14-11
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