ATG therapy differentially depletes T cells from peripheral blood and lymphoid organs. NOD mice were treated with ATG or isotype IgG twice with a 3-day interval. Then, CD4+ and CD8+T cells in peripheral blood were examined by flow cytometry every 3 days until day 22. A shows CD4+ and CD8+ T cell percentages in total peripheral white blood cells at different time points post ATG therapy (n=4 mice in each group); B and C show CD4+ and CD8+ T cell percentages in peripheral white blood cells and spleen cells, respectively (n=3 mice in each group). D exhibits CD4+ and CD8+ T cell depletion rates in peripheral blood and spleen at day 3 post ATG therapy, respectively (n=3 mice in each group). The similar results were obtained in additional two independent experiments.
Xia et al. BMC Immunology 2012 13:70 doi:10.1186/1471-2172-13-70