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New perspectives for natural antimicrobial peptides: application as antinflammatory drugs in a murine model

Rosanna Capparelli1*, Francesco De Chiara1, Nunzia Nocerino1, Rosa Chiara Montella1, Marco Iannaccone1, Andrea Fulgione1, Alessandra Romanelli2, Concetta Avitabile2, Giuseppe Blaiotta3 and Federico Capuano4

Author Affiliations

1 Faculty of Biotechnology, University of Naples “Federico II”, Naples, 80134, Italy

2 Department of Biological Sciences, University of Naples “Federico II”, Naples, 80134, Italy

3 Department of Food Science, University of Naples “Federico II”, Portici, 80055, Italy

4 Department of Food Inspection IZS ME, via Salute 2, Portici, 80055, Italy

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BMC Immunology 2012, 13:61  doi:10.1186/1471-2172-13-61

Published: 17 November 2012

Additional files

Additional file 1:

Table S1. Lethal concentration (LC50) of Temporin B –KK, Royal jelleins-IC, MIX through their hemolytic activity on mouse erythrocytes.

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Additional file 2:

Figure S1. Anti-inflammatory activity. The levels of IFN- γ and TNF- α were determined by a sandwich ELISA test in J774 cells untreated; J774 cells infected with S. epidermidis for 1 h; J774 cells stimulated with RJI-C (9 μg/ml) for1 h; J774 cells stimulated with TB-KK. (6 μg/ml) for1 h; J774 cells stimulated with MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) for1 h; J774 cells infected with S. epidermidis for 1 h and stimulated with MIX for 1 h. Results from two representative experiments are presented as mean value ± S.D. *P <0.05, **p < 0.01; ***p < 0.001, Student’s t test S. epidermidis vs S. epidermidis + MIX.

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Additional file 3:

Figure S2 (A-B). Bacterial load in spleen and kidneys of S.epidermidis infected mice (rumble line) and subsequently treated with the MIX (square line) or gentamicin (triangle line). Data are representative of 15 animals/group. Student’s t test gentamicin vs MIX not significant.

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Additional file 4:

Table S2. Acute phase proteins. Acute phase proteins from blood samples of mice infected with Staphylococcus epidermidis and treated with MIX or with Gentamicin.

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Additional file 5:

Figure S3. Time course (30, 60, 90 and 120 minutes) of bacterial load in blood of S. epidermidis infected mice.

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Additional file 6:

Table S3: Origin of S. epidermidis strains.

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Additional file 7:

Table S4: Sequences of the primers.

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