Analysis of Rag1 expression in lymphatic organs. RT-PCR of wild type, heterozygous and Rag1 mutant rats: The Rag1 PCR product (upper panel) was digested with StyI-HF to distinguish between translation of wild type and mutated Rag1 (mid panel). GAPDH used as housekeeping gene (lower panel). B Western Blot analysis: Translation of Rag1 in the thymus from wild type, heterozygous and Rag1 mutant rats compared to GAPDH as housekeeping protein. C Comparison of NK cells, B and T cell ratio in the peripheral blood of wild type, heterozygous and Rag1 mutated rats determined by FACS analysis. *** P < 0.0001; ** P < 0.005.
Zschemisch et al. BMC Immunology 2012 13:60 doi:10.1186/1471-2172-13-60