Analysis of the mutant Rag1 allele. A Heterozygosity at the genomic Rag1 locus of founder 58 was confirmed by sequencing. The start of the mixed signal indicated the Rag1 mutation identified as A to C exchange (green upper case) plus 4 bp deletion at the ZFN cut site (red lower case) and the ZFN binding regions (black upper case). The introduced frame shift caused a premature stop codon leading to a truncation of the Rag1 protein to a 198 aa N-terminal residue. B Full length Rag1 protein consists the functional important zinc-binding dimerization domain (ZDD) containing the RING finger (RING) and the zinc finger A (ZFA) as well as the c-terminal core domain with the nonamer-binding region (NBR) and the zinc finger B (ZFB). Mutated Rag1 lost all domains essential for Rag1 function in V(D)J recombination.
Zschemisch et al. BMC Immunology 2012 13:60 doi:10.1186/1471-2172-13-60