Additional file 2.

Figure S2. Illustration of the D-REAM method for identification of T-DMRs. D-REAM was developed via integration of a methylation-sensitive restriction enzyme (HpyCH4IV), ligation-mediated PCR (LM-PCR), and DNA tiling microarrays [3,4]. Briefly, for a certain genomic locus that contains a HpyCH4IV recognizing site (ACGT), in Sample A, the ACGT containing 5′-methylcytosine cannot be digested by HpyCH4IV and the corresponding fragment is not amplified by LM-PCR, thus does not detected by microarray. In contrast, in Sample B, the ACGT with unmethylated cytocine is digested and the corresponding fragment is efficiently amplified, leading to microarray signal, reflected by D-REAM score. Comparing the two samples using the D-REAM score, the locus is defined as a T-DMR and hypomethylated in Sample B. (PDF 242 kb)

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Wu et al. BMC Immunology 2012 13:58   doi:10.1186/1471-2172-13-58