Additional file 1.

Figure S1. Protocol for coronary/aortic inflammation in mice using CAWS. A full cycle of CAWS included two rounds of intra-peritoneal (I.P.) injections (1 mg/mouse/day for five consecutive days) administered four weeks apart as previously described47-51. Early experiments were conducted with a dose of 4 mg instead of 1 mg of CAWS per day. Results derived from either dose had identical disease incidence and severity. In some experiments, mice were sacrificed 10 days after the first five days of CAWS injections (cycle one). At this time point we were unable to identify ongoing inflammation in the coronary or aortic walls (data not shown). We identified inflammation clearly and consistently in 100% of mice 30 days after the completion of the first cycle.

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Martinez et al. BMC Immunology 2012 13:56   doi:10.1186/1471-2172-13-56