Decreased inflammatory infiltrate in the hearts of Ccr2−/− mice injected with CAWS. (A) Immunohistochemical staining of macrophages using the marker ER-HR3 in Ccr2+/+ mice and (B) quantification (pixels) of infiltration, at the level of the root of the aorta. (C) Absolute number of T cells (CD4+). (D) Representative FACS plots of neutrophils as defined by the expression of CD11b+ and Gr-1+ in Ccr2+/+ and (E) Ccr2−/− mice, with (F) absolute number of neutrophils. (G) FACS of iMo (CD11b+, Ly6Chi, Ly6Gint) in Ccr2+/+ mice and (H) Ccr2−/− mice, with (I) absolute number of iMo. (J) Representative plots of dendritic cells (CD11b+, CD11c+, I-Ab+) in Ccr2+/+ mice and (K) Ccr2−/− mice. (L) Absolute number of dendritic cells. A-L represents data collected from the heart at the level of the aortic root after two cycles of CAWS. Each bar represents the mean ± SE from a representative experiment with 6–8 mice per group.
Martinez et al. BMC Immunology 2012 13:56 doi:10.1186/1471-2172-13-56