Simultaneous co-expression of TGFß and IL10 in CD4+CD25+IFNγ+Foxp3+and CD4+CD25+IFNγ+CD127-iTreg. PBL of 3 healthy control individuals were stimulated for 16 h using PMA/Ionomycin. Simultaneous co-expression of TGFß and IL10 in CD4+CD25+IFNγ+Foxp3+ and CD4+CD25+IFNγ+CD127- iTreg was determined using six-color flow cytometry. (a, b) 0.5% of CD4+CD25+IFNγ+Foxp3+ and 0.7% of CD4+CD25+IFNγ+CD127- iTreg co-expressed the immunosuppressive cytokines TGFß as well as IL10 intracellularly, and 5% of CD4+CD25+IFNγ+Foxp3+ and 14% of CD4+CD25+IFNγ+CD127- iTreg produced TGFß but not IL10 (all percentages with respect to total of CD4+ PBL in the cell culture). Data are given as mean±SD. Unstim = incubated in medium, stimulated = incubated in PMA/Ionomycin.
Daniel et al. BMC Immunology 2012 13:47 doi:10.1186/1471-2172-13-47