Co-expression of CD178, CD152, CD279, CD28, CD95, HLA-DR, IL4, IL10, and TGFß on/in CD4+CD25+Foxp3+CD127-IFNγ+iTreg. PBL of 3 healthy control individuals were stimulated for 16 h using PMA/Ionomycin. Lymphocytes were determined in a FSC/SSC dot plot using six-color-fluorescence flow cytometry. Gates for fluorochrome-labeled monoclonal antibodies were defined using appropriate isotype controls that were adjusted to <1% positive cells in the corresponding quadrants. Based on these isotype controls, CD4+CD25+Foxp3+CD127-IFNγ+ PBL were determined and co-expression of CD178, CD152, CD279, CD28, CD95, HLA-DR, IL4, IL10, and TGFß was analyzed. Figure (a) depicts the gating strategy, Figure (b) summarizes the results of the 3 healthy controls. Data are given as mean±SD. Unstim = incubated in medium, stimulated = incubated in PMA/Ionomycin.
Daniel et al. BMC Immunology 2012 13:47 doi:10.1186/1471-2172-13-47