Additional file 1.

Optimized PCR condition for Rag2 and TREC genomic DNA amplification.A, Titration curves of cloned Rag2 and TREC templates of known concentrations. The standardization condition was applied to create a log dilution series for each assay. The coefficient of correlation for Rag2 were R = 0.9993, and for TREC R = 0.9992, indicating equal amplification of the templates over a range of input DNA concentrations. B, The equal and comparable slopes established for Rag2 and TREC PCR.

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Patel et al. BMC Immunology 2012 13:46   doi:10.1186/1471-2172-13-46