Vβ repertoire analysis of thein vitro-derived CD4 T cells. After anti-CD3/28 stimulation, the in vitro-derived CD4 T cells were surface stained for 24 Vβ families using the IOTest® Beta Mark TCR Vβ Repertoire Kit. CD3-gated population was evaluated for the expression of Vβ families of protein. A, TCR Vβ analysis of in vitro developed CD4 T cells of five CD34+ HPC donors versus a control PBMC. B, Summary analysis of Vβ distributions of four control PBMCs versus five in vitro derived CD4 T cells.
Patel et al. BMC Immunology 2012 13:46 doi:10.1186/1471-2172-13-46