Flow cytometry analyses of expression kinetics of CD3, TCRαβ and TCRγδ of the developing T cells under LmDL1 + IL-7 + Flt3L or LmDL1-FL7 co-culture condition. Schematic illustration of key stages of T cell development is shown at top. T cell markers CD3, TCRαβ, and TCRγδ were analyzed for the developing T cells under the two different co-culture conditions up to 56 days. ND, not done, due to insufficient amount of developing T cells from the early time points of the LmDL1 + IL-7 + Flt3L coculture.
Patel et al. BMC Immunology 2012 13:46 doi:10.1186/1471-2172-13-46